Readiness of IT organisations to implement Artificial Intelligence to support business processes in Gauteng Province, South Africa.

Artificial Intelligence (AI) has emerged as a research field and more particularly studies pertaining to the readiness of organisations to implement AI. Although AI implementation has proliferated across industries, many organisations still struggle to successfully achieve their business goals, associated with AI and Fourth Industrial Revolution (4IR). This study attempts to close this gap, by conducting a deductive case study, and thematic analysis into the readiness of a Gauteng-based IT organisation to implement AI towards achieving their business goals, in line with the benefits associated with 4IR. To achieve this, the researcher draws on the Technology-Organisation-Environment (TOE) framework to reflect on the dimensions and group them into strategy, perception and awareness, challenges, and organisational culture, related to contextual factors. This paper reports on the outcomes of open-ended interviews and focus group discussions involving 31 participants across IT management, senior-, and junior technical staff, about the enabling and hindering factors of AI readiness. The study further offers insights and a research agenda to support IT managers and staff to make informed decisions towards increasing their readiness to implement A

Water and salt balances of two shallow groundwater cropping systems using subjective and objective irrigation scheduling

Evidence suggests that, in general, subjective rather than objective irrigation  scheduling decisions are adopted by farmers. Irrigators have ‘calibrated’ themselves with years of experience to irrigate subjectively according to perceived crop water requirements. This study aimed to determine the associated benefits of objective versus subjective scheduling of two shallow groundwater cropping systems. Weekly measurements included rainfall and irrigation amounts, soil water content, groundwater table depth, artificial drainage volumes, and electrical conductivity of irrigation water, groundwater and drainage water.  Simulations of evaporation and transpiration were done with the SWAMP model. Based on soil water and salinity status, matric and osmotic stress during the four cropping seasons is considered unlikely. When rainfall-plusirrigation was compared to evapotranspiration, objective scheduling resulted in an under-supply of 15%, and rainfall and shallow groundwater served as supplementary water sources. Subjective scheduling did not use rainfall efficiently as a source of water and resulted in an over-supply of 10%.  Approximately 50% less salt was leached with objective compared to subjective irrigation scheduling. Under shallow groundwater conditions, irrigating subjectively according to crop water requirement results in excessive irrigation, salt addition and leaching compared to objective scheduling. Farmers can address some of the environmental problems  associated with irrigation by adopting objective scheduling and reducing the leaching fraction (< 0.15) of shallow groundwater cropping  systems.Keywords: evapotranspiration, leaching, salinity, water conservation, water degradatio

Diagnosis and prevalence of persistent infection with bovine viral diarrhoea virus in South African feedlot cattle

Bovine viral diarrhoea virus (BVDV) infection is an important viral infection affecting the cattle industry today. The prevalence of this infection in South African feedlots is unknown. Ear notch biopsies were collected from chronic poor doers and animals that appeared unthrifty upon entering feedlots, as well as animals entering the hospital pen with respiratory disease for the first time. A total of 1690 samples were collected: 1074 from the former category and 616 from the latter. A routine immunohistochemistry staining protocol showed that 49 animals tested positive, of which 43 (4%) came from the feedlot entry group and six (1%) from the hospitalised group. The prevalence of persistently infected cattle from this selected, nonrandom sample entering six large South African feedlots was found to be 2.9%, which is higher than the international rule of thumb that 0.5% of all cattle entering feedlots are persistently infected. There was no clear correlation between persistent infection and respiratory disease. Serum samples were also collected when possible and 10 positive cases were found. Results from enzyme-linked immunosorbent assays for antigen and antibody performed on these sera correlated well with those from the immunohistochemistry staining method in six cases, but in four cases the animals tested falsely positive owing to nonspecific staining. Immunohistochemistry staining on ear notch biopsies is thus a reliable diagnostic method to identify persistently infected animals with BVDV, but the pathologist should be aware of nonspecific positive staining.http://www.ojvr.orgmn201

Heterologous expression of the class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, in escherichia coli using green fluorescent protein as a fusion partner

CITATION: Vermeulen, R. R., Du Preez Van Staden, A. & Dicks, L. 2020. Heterologous expression of the class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, in escherichia coli using green fluorescent protein as a fusion partner. Frontiers in Microbiology, 11:1634, doi:10.3389/fmicb.2020.01634.The original publication is available at https://www.frontiersin.orgPublication of this article was funded by the Stellenbosch University Open Access FundThe antilisterial class IIa bacteriocins, plantaricin 423 and mundticin ST4SA, have previously been purified from the cell-free supernatants of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA, respectively. Here, we present the fusions of mature plantaricin 423 and mundticin ST4SA to His-tagged green fluorescent protein (GFP) for respective heterologous expression in Escherichia coli. Fusion of plantaricin 423 and mundticin ST4SA to His-tagged GFP produced the fusion proteins GFP-PlaX and GFP-MunX, respectively. Both fusion proteins were autofluorescent, circumvented inclusion body formation and lowered the toxicity of class IIa bacteriocins during heterologous expression. Not only did GFP-class IIa fusion stabilize heterologous expression and boost yields, the fluorescent intensity of GFP-PlaX and GFP-MunX could be monitored quantitatively and qualitatively throughout expression and purification. This robust fluorometric property allowed rapid optimization of conditions for expression and bacteriocin liberation from GFP via the incorporated WELQut protease cleavage sequence. Incubation temperature and IPTG concentration had a significant effect on bacteriocin yield, and was optimal at 18°C and 0.1–0.2 mM, respectively. GFP-MunX was approximately produced at a yield of 153.30 mg/L culture which resulted in 12.4 mg/L active mundticin ST4SA after liberation and HPLC purification. While GFP-PlaX was produced at a yield of 121.29 mg/L culture, evidence suggests heterologous expression resulted in conformation isomers of WELQut liberated plantaricin 423.https://www.frontiersin.org/articles/10.3389/fmicb.2020.01634/fullPublisher's versio

Nodal-positives Prostatakarzinom : Stellenwert der zytoreduktiven radikalen Prostatektomie

All South African universities face the challenges of student success and retention - a challenge compounded by under-prepared students from diverse backgrounds. This article explores peer module mentoring as a possible approach to facilitate student success in Economic and Management Sciences. An ex post facto quantitative research methodology, with a web-based questionnaire, was implemented. Perspectives of both mentors and mentees who participated in the faculty module mentoring programme during 2012 were investigated regarding their motivation for participation and evaluation of the programme. The module mentoring programme was experienced as being beneficial by both the mentors and mentees. Altruistic, cognitive, social, personal growth and financial benefits were derived from the programme. The findings provide a strong argument in favour of the expanding and continuation of module mentoring programmes in the Faculty of Economic and Management Sciences. The findings are not generalisable beyond the scope of the particular faculty and institution, but provide input and guidance for decision-making related to student support initiatives. The continuance of the module mentoring programme entails investments in terms of both money and time. This article considers the benefits derived from these investments in a faculty-wide module mentoring programme at a South African university

Parasite host-switching from the invasive American red-eared slider, Trachemys scripta elegans, to the native Mediterranean pond turtle, Mauremys leprosa, in natural environments

The red-eared slider turtle, Trachemys scripta elegans, is among the most over-exploited animals and is still exported annually from the USA all over the world. Once introduced into its new environment, feral populations may arise and pose threats to local biodiversity and ecosystem functioning. In France, it is in fact considered as a risk for the Mediterranean pond turtle, Mauremys leprosa, and the European pond turtle, Emys orbicularis, as they may compete for resources and habitat. Freshwater turtles are also host to a variety of parasites including protozoans and helminths. When introduced turtles escape, parasites may spread to native species. The objective of this study was to document the extent of platyhelminth invasions from T. s. elegans to natural M. leprosa populations in northern Spain and southern France and to evaluate the risks that parasite host-switching may pose on indigenous freshwater turtle species. From DNA barcoding analysis based on the sequencing of the Cytochrome c Oxidase I gene, the Bayesian tree and p-distance comparisons of closely related haplotypes revealed a greater polystome richness within M. leprosa than expected, suggesting that host switching may take place in natural environments. Because these parasites most typically infest American turtles like Chrysemys picta marginata and Graptemys pseudogeographica in their natural home range and because parasites were also found within T. s. elegans feral populations, it is suggested that the red-eared slider would serve as a carrier for a variety of not strictly host-specific polystomes that are transmitted to M. leprosa throughout the south of France. The global trade in freshwater turtles thus provides opportunity for parasites to be transported to new destinations which could impact the physiology, behavior and survival of native turtle specie

Parasite host-switching from the invasive American red-eared slider, Trachemys scripta elegans, to the native Mediterranean pond turtle, Mauremys leprosa, in natural environments

International audienceThe red-eared slider turtle, Trachemys scripta elegans, is among the most over-exploited animals and is still exported annually from the USA all over the world. Once introduced into its new environment, feral populations may arise and pose threats to local biodiversity and ecosystem functioning. In France, it is in fact considered as a risk for the Mediterranean pond turtle, Mauremys leprosa, and the European pond turtle, Emys orbicularis, as they may compete for resources and habitat. Freshwater turtles are also host to a variety of parasites including protozoans and helminths. When introduced turtles escape, parasites may spread to native species. The objective of this study was to document the extent of platyhelminth invasions from T. s. elegans to natural M. leprosa populations in northern Spain and southern France and to evaluate the risks that parasite host-switching may pose on indigenous freshwater turtle species. From DNA barcoding analysis based on the sequencing of the Cytochrome c Oxidase I gene, the Bayesian tree and p-distance comparisons of closely related haplotypes revealed a greater polystome richness within M. leprosa than expected, suggesting that host switching may take place in natural environments. Because these parasites most typically infest American turtles like Chrysemys picta marginata and Graptemys pseudogeographica in their natural home range and because parasites were also found within T. s. elegans feral populations, it is suggested that the red-eared slider would serve as a carrier for a variety of not strictly host-specific polystomes that are transmitted to M. leprosa throughout the south of France. The global trade in freshwater turtles thus provides opportunity for parasites to be transported to new destinations which could impact the physiology, behavior and survival of native turtle species

Data from: A revision of African helmeted terrapins (Testudines: Pelomedusidae: Pelomedusa), with descriptions of six new species

Using nearly range-wide sampling, we analyze up to 1848 bp of mitochondrial DNA of 183 helmeted terrapins and identify a minimum of 12 deeply divergent species-level clades. Uncorrected p distances of these clades equal or clearly exceed those between the currently recognized species of Pelusios, the genus most closely related to Pelomedusa. We correlate genetic discontinuities of Pelomedusa with data on morphology and endoparasites and describe six new Pelomedusa species. Moreover, we restrict the name Pelomedusa subrufa (Bonnaterre, 1789) to one genetic lineage and resurrect three further species from its synonymy, namely P. galeata (Schoepff, 1792), P. gehafie (Rüppell, 1835), and P. olivacea (Schweigger, 1812). In addition to these ten Pelomedusa species, we identify two further clades from Cameroon and Sudan with similar levels of genetic divergence that remain unnamed candidate species. We also note that some problematical terrapins from South Africa and Somalia may represent two additional candidate species. Some of the Pelomedusa species are morphologically distinctive, whilst others can only be identified by molecular markers and are therefore morphologically cryptic taxa

Pelomedusa_Supporting-Information_Table_S1

TABLE S1. Used samples, GenBank sequences and their accession numbers. Mitochondrial lineages sensu Vargas-Ramírez et al. (2010) and Fritz et al. (2014) indicated